Immunochemical properties of anti-cord factor antibody.
نویسنده
چکیده
Sera of man and animals infected with tubercle bacilli were tested for precipitating antibody to trehalose-6, 6'-dimycolate (cord factor) of Mycobacterium tuberculosis. None of the sera of mice and rabbits infected with M. tuberculosis H37Rv reacted with cord factor, whereas the sera of animals vaccinated with cord factor-methylated bovine serum albumin complex demonstrated a precipitin reaction with cord factor. Antibody against cord factor was not detected in the sera of cases of active, convalescent, and pneumonectomized human tuberculosis, indicating that the anti-cord factor antibody is not associated with tuberculous infection in man and animals. Precipitin activity against cord factor was localized in 19S macroglobulin (immunoglobulin M) fraction of the serum of rabbits vaccinated with cord factor-methylated bovine serum albumin complex. Analytical gel filtration and immunodiffusion studies indicated that the anti-cord factor precipitin dissociated from the cord factor-antibody complex is an immunoglobulin M antibody.
منابع مشابه
Effect of anti-cord factor antibody on experimental tuberculosis in mice.
Either active immunization with trehalose-6, 6'-dimycolate (cord factor)-methylated bovine serum albumin complex or passive transfer of rabbit anti-cord factor serum induced in mice an enhanced resistance against infection with virulent human Mycobacterium tuberculosis. This suggests that the anti-cord factor antibody exerts an infection-protecting effect by neutralizing the toxic action of cor...
متن کاملتولید و تخلیص آنتی بادی پلی کلونال بر علیه هورمون ملاتونین
Nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. Producing antibody against immunogenes is the first step to make immunochemical kits. In this study production and purification of polyclonal antibody against melatonin has been considered. This hormone which has several important functions in...
متن کاملThe idiotypic characteristics of human antibodies to factor VIII.
We have prepared an antiidiotype antibody that specifically reacts with and inactivates a human autoantibody to factor VIII (VIII:C). The antiidiotype specificity of this rabbit antibody was evident in solution, as specific inhibition of anti-VIII:C activity, and in solid-phase studies. The antiidiotype antibody inhibited both plasma anti-VIII:C and Fab' fragments prepared from that plasma's Ig...
متن کاملHigh Serum Levels of Rheumatoid Factor and Anti-Phosphatidylserine Antibody in Patients with Ischemic Heart Disease
Background: Immunopathological and inflammatory processes play important roles in the initiation and development of Ischemic Heart Disease (IHD). Objective: The aim of this study was to evaluate the serum levels of several autoantibodies including rheumatoid factor (RF), anti-nuclear antibodies (ANA), anti-small nuclear ribonucleoprotein (anti-Sm), anti-phosphatidylserine (anti-PS) and anti-car...
متن کاملExtraction and Purification of Anti-Proteinase 3 (PR3) Antibodies from Egg Yolk
Recently it has been reported, that immunoglobulin Y (IgY) can be used instead of polyclonal antibodies extracted from mammals (IgG) for the purpose of diagnosis and therapy. These antibodies are found to have better properties in terms of specificity and ease of large-scale production. In addition, IgY binds neither to mammalian complement or Fc-receptors nor does it interfere with rheumatoid ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Infection and immunity
دوره 7 1 شماره
صفحات -
تاریخ انتشار 1973